复方861对大鼠肝脏卵圆细胞分化的影响

目的探讨复方861对大鼠肝脏卵圆细胞分化的影响,了解其在肝纤维化治疗过程中促进肝细胞再生的可能机制。方法不同浓度(1.95,3.90,7.81,15.62,31.25,62.50,125,250,500,1000μg/mL)的复方861在无血清培养条件下作用于WB-F344细胞24 h,MTT法分析法检测细胞生长情况。500μg/mL复方861在无血清条件下作用WB-F344细胞72 h后,通过RT-PCR观察CK-19、AFP、ALB、αmRNA表达的变化。以同期未作处理的WB-F344作为空白对照组。结果 WB-F344细胞经过不同复方861作用后,除1000μg/mL外,各组细胞生长均未受到抑制,500μg/mL时细胞生存活性最佳。无血清条件下作用72 h后,半定量RT-PCR发现861组AFP mRNA的表达显著增加,CK-19 mRNA的表达显著减少,同时发现861组有ALB mRNA的表达。结论复方861可能诱导WB-F344细胞主要向肝细胞方向分化。

The effect of Cpd861 on the differentiation of hepatic oval cells in rats

Objective To investigate the effect of Cpd861 （ Compound 861, Cpd861 ） on the differentiation of rat liver oval cell line WB-F344 in vitro. Method WB-F344 ceils were treated with Cpd861 （ 1. 95, 3.90, 7.81, 15.62, 31.25, 62.5,125,250, 500, 1000μg/mL） in the serum-free medium for 72 hours. The cell growth was measured by MTT analysis. The mRNA levels of some phenotypic markers, such as CK19, AFP and ALB were determined by RT-PCR. Untreated WB-F344 cells were used as blank control. Result We found that Cpd861 did not inhibit the growth of WB- F344 cells from 1.95 to 500 μg/mL. WB-F344 cells treated with 500 μg/mL Cpd861 in the absence of any serum. After 3 d, RT-PCR showed increased expression of AFP in Cpd treated groups; the expression of CK19 was lower than that of control group. ALB expression was observed in Cpd861 treated ceils but not in blank control group. Conclusion Cpd861 might induce WB-F344 cells differentiation into the hepatic lineage in vitro.