|
|||||
|
|
| 针对乙肝病毒的串联序列amiRNA表达载体的构建 | |
| 引用本文： | 蒲春文,王莉芬,赵亮,曲素丽,张影,孙伟祥,黄鑫,程宁.针对乙肝病毒的串联序列amiRNA表达载体的构建[J].中国微生态学杂志,2011,23(10):902-905,908. |
| 作者姓名： | 蒲春文 王莉芬 赵亮 曲素丽 张影 孙伟祥 黄鑫 程宁 |
| 作者单位： | 1. 大连市第六人民医院,辽宁大连 116000;大连医科大学,辽宁大连 116044 2. 大连医科大学附属二院,辽宁大连,116027 3. 大连市第六人民医院,辽宁大连,116000 4. 大连市医学会,辽宁大连,116000 |
| 摘 要： | 目的为优化RNA干扰研究方法,构建了针对乙肝病毒的串联序列amiRNA(artificial microRNA)质粒表达载体。方法设计针对乙型肝炎病毒S区的靶干扰序列,构建单一序列amiRNA质粒表达载体;将其中干扰效率好的两个序列串联起来,构建串联序列amiRNA质粒表达载体。结果经酶切及测序鉴定,插入序列与靶序列一致,载体构建正确。结论成功构建了新型针对乙肝病毒的串联序列amiRNA质粒表达载体,为进一步体外及体内实验奠定了基础。 |
| 关 键 词： | 乙型肝炎病毒 RNA干扰 amiRNA 串联序列质粒表达载体 |
Construction of the expression plasmids of tandem sequence amiRNA against HBV |
|
| PU Chun-wen,WANG Li-fen,ZHAO Liang,QU Su-li,ZHANG Ying,SUN Wei-xiang,HUANG Xin,CHENG Ning.Construction of the expression plasmids of tandem sequence amiRNA against HBV[J].Chinese Journal of Microecology,2011,23(10):902-905,908. | |
| Authors: | PU Chun-wen WANG Li-fen ZHAO Liang QU Su-li ZHANG Ying SUN Wei-xiang HUANG Xin CHENG Ning |
| Institution: | PU Chun-wen1,2,WANG Li-fen3,ZHAO Liang1,QU Su-li4,ZHANG Ying1,SUN Wei-xiang1,HUANG Xin1,CHENG Ning1 (1.The Sixth People's Hospital of Dalian,Dalian 116000,China,2.Dalian Medical University,Dalian 116044,3.The Second Affiliated Hospital of Dalian Medical University,Dalian 116027,4.Dalian Medical Association,China) |
| Abstract: | Objective To optimize the design of RNA interference and construct the tandem sequence amiRNA expression plasmids against HBV. Method Aiming at the conservation sites,singular-sequence vectors amiRNA-HBV1,amiRNA-HBV2,amiRNA-HBV3 and amiRNA-HBV4 were constructed.These vectors were transfected into HepG2.2.15 transiently.The two sequences with high efficiency were chosen,and then the tandem-sequence vector amiRNA HBV3-HBV4 was built. Result Four constructed miRNA expression vectors and the tandem one were ver... |
| Keywords: | HBV RNAi AmiRNA Tandem-sequence vector |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |