| 产淀粉酶益生乳杆菌的筛选及鉴定 |
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| 引用本文： | 赵志丹,郭小华,熊海容. 产淀粉酶益生乳杆菌的筛选及鉴定[J]. 中国微生态学杂志, 2011, 23(9): 769-773,779 |
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| 作者姓名： | 赵志丹 郭小华 熊海容 |
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| 作者单位： | 中南民族大学微生物与生物转化重点实验室,湖北武汉,430074 |
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| 基金项目： | 国家自然科学基金，教育部留学归国人员科研启动基金，中南民族大学自然科学基金重点项目 |
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| 摘 要： | 目的以健康仔猪肠道及粪便样品为基础,从中筛选产淀粉酶的乳杆菌菌株,并评价其作为益生菌候选菌株潜力。方法用MRS培养基分别从仔猪新鲜粪便和小肠黏膜上分离到乳酸菌,采用改良的产淀粉酶选择性培养基初筛得到能降解淀粉活性的菌株,并研究菌株的淀粉水解活性、抗逆能力、粘附特性及对抗生素的敏感性。结果从备选的485株乳酸菌中筛选得到具有初步淀粉酶活性的菌株25株（占总筛选数量的5.2%）,复筛选育得到具有较强淀粉酶活性的乳杆菌3株。进一步研究了这3株乳杆菌的抗逆能力、粘附特性以及对抗生素的耐药性,并对最终选育得到的菌株进行生理生化及16S rRNA分子鉴定。经选育鉴定的罗伊乳杆菌G8-5淀粉降解能力最强,并能耐受pH 3.0的酸度、1.0%的胆盐浓度,在小肠上皮细胞上的粘附效率超过15个以上,并对常用的抗生素具有较高的敏感率。结论罗伊乳杆菌G8-5符合安全益生菌的要求,可以作为产淀粉酶的益生乳杆菌优良的候选菌株。
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| 关 键 词： | 乳杆菌 筛选 淀粉酶活性 特性 |
Screening of Lactobacillus with amylolytic activity and its identification |
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| ZHAO Zhi-dan,GUO Xiao-hua,XIONG Hai-rong. Screening of Lactobacillus with amylolytic activity and its identification[J]. Chinese Journal of Microecology, 2011, 23(9): 769-773,779 |
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| Authors: | ZHAO Zhi-dan GUO Xiao-hua XIONG Hai-rong |
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| Affiliation: | ZHAO Zhi-dan,GUO Xiao-hua,XIONG Hai-rong(Key Lab for Microorganisms and Biotransformation,South-Central University for Nationalities,Wuhan 430070,China) |
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| Abstract: | Objective To screen Lactobacillus strains from the intestinal mucus and fresh feces of healthy piglets in order to develop probiotics with amylolytic activity.Method 485 strains were isolated from the fresh feces and intestinal mucus of piglets based on selective MRS media.Result 25 strains(5.2%) with amylolytic activity were screened based on modified amylase selective media,3 strains of which were verified as having high amylolytic activity.The 3 strains were further studied for their basic probiotic acti... |
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| Keywords: | Lactobacillus Screening Amylolytic activity Characteristics |
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