广州地区人腺病毒5型血清流行病学初步调查

目的评估广州地区人群中人腺病毒5型(HAdV-5)先存中和抗体的流行情况。方法采用以β-半乳糖苷酶(LacZ)为报告基因,结合CMV启动子的人重组腺病毒5型,使用化学发光法,检测了209份免疫功能正常的成人血清样本中HAdV-5的中和抗体阳性率情况。结果 HAdV-5中和抗体的阳性率为82.3%(172/209)。HAdV-5中和抗体在<1/20(低滴度)、1/40-1/160(中滴度)、1/320-1/1280(高滴度)、>1/1280(超高滴度)时均能检测到,而且随着滴度的增加,阳性率逐渐升高。在20～40岁时HAdV-5中和抗体阳性率最高,在<20岁时HAdV-5阳性率最低。结论广州地区人群中针对HAdV-5的先存中和抗体阳性率高,应用基于该种DNA病毒作为载体进行基因疫苗及基因治疗的研究时,具有一定的局限性。

The preliminary investigation of seroepidemiology of Human adenovirus type 5 in Guangzhou

Objective To estimate the epidemic situation of preexisting neutralizing antibody of human adenovirus type 5（HAdV-5） in Guangzhou.Method β-galactosidase（LacZ） was used as the reporter gene,combined with the genome of HAdV-5 driven by CMV promoter.Then chemiluminescence method was used to investigate the ratio of positive neutralizing antibodies of HAdV-5 in the sera of 209 immunocompetent individuals.Result The ratio of positive neutralizing antibodies of HAdV-5 was 82.3%（172/209）.The HAdV-5 neutralizing antibodies were found at low（1/20）,medium（1/40-1/160）,high（1/320-1/1280） and ultra-high（1/1280） titers.And the ratio of positive gradually increased as the titer increased.The antibodies were highly prevalent at high and ultra-high titers.The highest rate of HAdV-5 neutralizing antibodies appeared at the ages of 20 to 40,while the lowest rate was seen in ages under 20.Conclusion The neutralizing antibodies against HAdV-5 have a high positive ratio in Guangzhou population.So there are some limitations using this virus vector for gene vaccine and gene therapy research.