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广州地区人腺病毒5型血清流行病学初步调查
引用本文:卢建溪,梅明珠,钱师宇,阳莉,李刚. 广州地区人腺病毒5型血清流行病学初步调查[J]. 中国微生态学杂志, 2011, 23(9): 826-828
作者姓名:卢建溪  梅明珠  钱师宇  阳莉  李刚
作者单位:1. 中山大学附属第三医院疫苗研究所,广东广州,510630
2. 暨南大学医学院,广东广州,510632
3. 中山大学附属第三医院感染科,广东广州,510630
基金项目:国家高技术研究发展计划(863计划),广东省自然科学基金资助项目,广东省科技计划资助项目
摘    要:目的评估广州地区人群中人腺病毒5型(HAdV-5)先存中和抗体的流行情况。方法采用以β-半乳糖苷酶(LacZ)为报告基因,结合CMV启动子的人重组腺病毒5型,使用化学发光法,检测了209份免疫功能正常的成人血清样本中HAdV-5的中和抗体阳性率情况。结果 HAdV-5中和抗体的阳性率为82.3%(172/209)。HAdV-5中和抗体在<1/20(低滴度)、1/40-1/160(中滴度)、1/320-1/1280(高滴度)、>1/1280(超高滴度)时均能检测到,而且随着滴度的增加,阳性率逐渐升高。在20~40岁时HAdV-5中和抗体阳性率最高,在<20岁时HAdV-5阳性率最低。结论广州地区人群中针对HAdV-5的先存中和抗体阳性率高,应用基于该种DNA病毒作为载体进行基因疫苗及基因治疗的研究时,具有一定的局限性。

关 键 词:人腺病毒5型  化学发光法  中和抗体

The preliminary investigation of seroepidemiology of Human adenovirus type 5 in Guangzhou
LU Jian-xi,MEI Ming-zhu,QIAN Shi-yu,YANG Li,LI Gang. The preliminary investigation of seroepidemiology of Human adenovirus type 5 in Guangzhou[J]. Chinese Journal of Microecology, 2011, 23(9): 826-828
Authors:LU Jian-xi  MEI Ming-zhu  QIAN Shi-yu  YANG Li  LI Gang
Affiliation:LU Jian-xi1,MEI Ming-zhu1,QIAN Shi-yu2,YANG Li1,LI Gang3(1.Vaccine Research Institute,the Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510630,China,2.School of Medicine,Jinan University,Guangzhou 510632,3.Department of Infectious Disease,China)
Abstract:Objective To estimate the epidemic situation of preexisting neutralizing antibody of human adenovirus type 5(HAdV-5) in Guangzhou.Method β-galactosidase(LacZ) was used as the reporter gene,combined with the genome of HAdV-5 driven by CMV promoter.Then chemiluminescence method was used to investigate the ratio of positive neutralizing antibodies of HAdV-5 in the sera of 209 immunocompetent individuals.Result The ratio of positive neutralizing antibodies of HAdV-5 was 82.3%(172/209).The HAdV-5 neutralizing antibodies were found at low(1/20),medium(1/40-1/160),high(1/320-1/1280) and ultra-high(1/1280) titers.And the ratio of positive gradually increased as the titer increased.The antibodies were highly prevalent at high and ultra-high titers.The highest rate of HAdV-5 neutralizing antibodies appeared at the ages of 20 to 40,while the lowest rate was seen in ages under 20.Conclusion The neutralizing antibodies against HAdV-5 have a high positive ratio in Guangzhou population.So there are some limitations using this virus vector for gene vaccine and gene therapy research.
Keywords:Human adenovirus  Chemiluminescence  Neutralizing antibody  
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