Engineering of choline oxidase from Arthrobacter nicotianae for potential use as biological bleach in detergents |
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Authors: | Doris Ribitsch Sonja Winkler Karl Gruber Wolfgang Karl Eva Wehrschütz-Sigl Inge Eiteljörg Petra Schratl Peter Remler Regina Stehr Cornelius Bessler Nina Mußmann Kerstin Sauter Karl Heinz Maurer Helmut Schwab |
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Institution: | 1. Austrian Centre of Industrial Biotechnology (ACIB), c/o Research Centre Applied Biocatalysis, Petersgasse 14, 8010, Graz, Austria 3. Institute of Molecular Biosciences, University of Graz, Humboldtstrasse 50/3, 8010, Graz, Austria 4. Henkel AG & Co. KGaA, Henkelstrasse 67, 40191, Duesseldorf, Germany 2. Institute of Molecular Biotechnology, Graz University of Technology, Petersgasse 14, 8010, Graz, Austria
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Abstract: | In order to engineer the choline oxidase from Arthrobacter nicotianae (An_CodA) for the potential application as biological bleach in detergents, the specific activity of the enzyme toward the synthetic substrate tris-(2-hydroxyethyl)-methylammonium methylsulfate (MTEA) was improved by methods of directed evolution and rational design. The best mutants (up to 520% wt-activity with MTEA) revealed mutations in the FAD- (A21V, G62D, I69V) and substrate-binding site (S348L, V349L, F351Y). In a separate screening of a library comprising of randomly mutagenised An_CodA, with the natural substrate choline, four mutations were identified, which were further combined in one clone. The constructed clone showed improved activity towards both substrates, MTEA and choline. Mapping these mutation sites onto the structural model of An_CodA revealed that Phe351 is positioned right in the active site of An_CodA and very likely interacts with the bound substrate. Ala21 is part of an α-helix which interacts with the diphosphate moiety of the flavin cofactor and might influence the activity and specificity of the enzyme. |
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