The effects of cyclophosphamide on in vitro cytotoxic responses to a syngeneic tumour |
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Authors: | Elizabeth J Hancock Douglas G Kilburn |
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Institution: | (1) Department of Microbiology, University of British Columbia, V6T 1W5 Vancouver, B.C., Canada |
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Abstract: | Summary We have studied the effects of treating DBA/2 mice with high doses of cyclophosphamide upon their subsequent ability to generate cytotoxic cells in vitro against syngeneic tumour antigens or alloantigens. High doses of cyclophosphamide (100–200 mg/kg body weight) eliminated the response to both antigens. The addition of normal DBA/2 thymocytes into these cultures restored the response to allogeneic cells but not to tumour cells. The anti-tumour response could be restored by the addition of interleukin 2 to the cultures. Treatment with high doses of cyclophosphamide decreased the number of anti-tumour cytotoxic cell precursors in the spleen, but did not affect the capacity of bulk cultures of spleen cells to produce interleukin 2 when stimulated with the mitogen concanavalin A.Abbreviations CY
Cyclophosphamide
- CTL
cytotoxic T cells
- CTLp
precursor cytotoxic T cells
- IL2
interleukin 2
- Con A
concanavalin A
- FCS
fetal calf serum |
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