Fructose-induced enhanced mitogenicity of diploid human cells: Possible relationship with cell differentiation |
| |
Authors: | C Wolfrom N Kadhom J Raulin N Raynaud M Gautier |
| |
Institution: | 1. INSERM U 56, H?pital de Bicêtre, F-94270, Kremlin-Bicêtre 2. Université Paris 7, Biologie Cellulaire, 2 Place Jussieu, F-75005, Paris
|
| |
Abstract: | Summary Fructose strongly stimulates the growth of normal diploid human skin fibroblasts (SFs) and induces marked changes in their
morphology and lipid accumulation. This mitogenic effect occurs despite very low fructose consumption and depends on the presence
of glutamine. The cell kinetics of cultured fructose-fed human skin fibroblasts were different from those fed on glucose:
in the presence of fructose a high proliferative index persisted at Day 14 of culture and the duration of the total cell cycle
and of the G1+1/2 M and S phases was slightly shorter. The mitogenic effect of fructose on SF was largest in the presence
of human serum: it was small or undetectable when fibroblasts were cultured in media supplemented with dialyzed human serum,
fetal bovine serum, or serum substitutes. This suggests that serum growth factor(s) mediate the mitogenic effect of fructose.
Only normal diploid human cells seem to be sensitive to this mitogenic effect of fructose: the long-term growth of normal
human liver cells on fructose was slightly better or similar to that on glucose. In contrast, fructose could only support
limited growth of hamster fibroblastic Nil cells and of a transformed human fibroblastic line, which grew better with glucose. |
| |
Keywords: | cell cycle fructose glutamine human fibroblasts human serum |
本文献已被 SpringerLink 等数据库收录! |
|