Molecular mechanism of null expression of aldehyde dehydrogenase-1 in rat liver |
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Authors: | James Chen Yuchio Yanagawa Akira Yoshida |
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Institution: | (1) Department of Biochemical Genetics, Beckman Research, Institute of the City of Hope, 1450 East Duarte Road, 91010 Duarte, California;(2) Present address: Department of Biochemistry, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan |
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Abstract: | In isozyme systems in general, the pattern of tissue-dependent expression of a given type of isozyme is uniform in various
mammalian species. In contrast, a major cytosolic aldehyde dehydrogenase isozyme, termed ALDH1, which is strongly expressed
in the livers of humans and other mammals, is hardly detectable in rat liver. Thirteen nucleotides existing in the 5′-promoter
region of human, marmoset, and mouseALDH1 genes are absent in the four rat strains examined. When the 13 nucleotides were deleted from a chloramphenicol acetyltransferase
expression construct, which contained the 5′-promoter region of the humanALDH1 gene and a low-background promoterless chloramphenicol acetyltransferase expression vector, the expression activity was severely
diminished in human hepatic cells. Thus, deletion of the 13 nucleotides in the promoter region of the gene can account for
the lack of ALDH1 expression in rat liver. |
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