Inhibition by adiponectin of IL-8 production by human macrophages upon coculturing with late apoptotic cells |
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Authors: | Saijo Shinji Nagata Kisaburo Nakano Yasuko Tobe Takashi Kobayashi Yoshiro |
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Affiliation: | Department of Biomolecular Science, Faculty of Science, Toho University, 2-2-1, Miyama, Funabashi, Chiba, Japan. |
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Abstract: | Adiponectin, an adipocyte-derived hormone, reportedly suppresses the production of TNF-alpha and IL-6 by LPS-stimulated human or porcine macrophages, and the phagocytosis of microbeads by human macrophages. In this study, we used a high molecular weight form of adiponectin purified from human plasma to examine its effects on the phagocytosis of late apoptotic cells by human macrophages and the subsequent IL-8 production. Adiponectin suppressed both the phagocytosis of apoptotic cells and the IL-8 production. In contrast, adiponectin augmented both the phagocytosis of apoptotic cells and the IL-8 production in the presence of LPS. These results suggest that adiponectin is not an anti-inflammatory hormone but rather a dual modulator of innate responses. |
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Keywords: | Human macrophages Phagocytosis IL-8 Apoptotic cells Adiponectin LPS CTLL-2 cells C1q Confocal microscopy Human plasma |
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