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The 26- and 14-kDa phosphoproteins associated with spinach chloroplast envelope membranes are distinct membrane-bound pools of the light-harvesting complex of photosystem II and of the small subunit of ribulose-1,5-bisphosphate carboxylase-oxygenase
Authors:L Bovet  M O Müller  P A Siegenthaler
Institution:(1) Laboratoire de Physiologie Végétale, Université de Neuchâtel, Rue Emile-Argand 13, CH-2007 Neuchâtel, Switzerland
Abstract:Two chloroplast envelope proteins from spinach (Spinacia oleracea L.) exhibiting relative molecular masses (Mrs) of 26 and 14 kDa are apparently phosphorylated by a unique Ca2+-dependent serine protein kinase. The activity of this enzyme shows the same sensitivity towards pH, Ca2+, Mg2+, H7 1-(5-isoquinolinesulphonyl)-2-methylpiperazine] and ATP concentrations (Siegenthaler and Bovet 1993, Planta 190, 231–240). Autoradiographic analyses following two-dimensional-gel electrophoresis (isoelectric focusing and SDS-PAGE) associated with Western blotting experiments indicate that these two phosphoproteins appeared to be pools of the light-harvesting complex of photosystem II (LHCII) and of the ribulose-1,5-bisphosphate carboxylase-oxygenase (Rubisco) small subunit, respectively. Immunoprecipitation of envelope-phosphorylated proteins, using immunoglobulins (IgG) directed to the apoprotein of LHCII and to the holoenzyme of Rubisco confirmed that LHCII and the Rubisco small subunit effectively incorporated 32P from (gamma-32P)ATP in isolated envelope membranes. We propose that, in agreement with the fact that protein import is driven by ATP, the phosphorylation of LHCII and the Rubisco small subunit could take place after the processing of precursor proteins and could be an obligatory step for their internalization into chloroplasts.Abbreviations 2D two dimensional - IEF isoelectric focusing - IgG immunoglobulin G - LHCII light-harvesting chlorophyll a/b proteins of PSII - LHCII A apoprotein a of LHCII - LHCIIB apoprotein b of LHCII - LS Rubisco large subunit - Mops (3-N-morpholino]propanesulfonic acid) - Mr relative molecular mass - PI isoelectric point - Rubisco ribulose-1,5-bisphosphate carboxylase-oxygenase - SS Rubisco small subunit The authors are grateful to Delphine Herrmann and Xavier Denys for their technical assistance. They also greatly thank Prof. R. J. Ellis and Dr. L. Barnett (Warwick University, UK) and Dr. P. Schürmann (University of Neuchâtel, Switzerland) for providing them with antibodies directed to the pea and spinach Rubisco holoenzymes and Dr. M. Spangfort (Lund University, Sweden) for his gift of the antibody directed to the pea LHCII apoprotein. This study was supported by the Swiss National Science Foundation. This work was part of a doctoral program carried out by L.B. in the Laboratoire de Physiologie végétale, Université de Neuchâtel, Switzerland.
Keywords:Chloroplast envelope  Light-harvesting chlorophyll a/b protein of PSII  Phosphoprotein  Protein kinase  Ribulose-1  5-bisphosphate carboxylase-oxygenase (small subunit)  Spinacia
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