STUDIES ON PROTEIN METABOLISM IN HIGHER PLANTS. III. CHANGES IN THE SOLUBLE PROTEIN COMPONENTS WITH LEAF GROWTH |
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Authors: | KAWASHIMA, NOBUMARO IMAI, AYAKO TAMAKI, EINOSUKE |
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Affiliation: | Division of Plant Biochemistry, Central Research Institute, Japan Monopoly Corporation Nishi-shinagawa, Shinagawa-ku, Tokyo |
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Abstract: | Water soluble protein was extracted from tobacco leaves (BrightYellow) and fractionated through chromatographic and immunochemicalprocedures. Six different UV-absorbing components, 7 antigeniccomponents, and 4 enzyme activities (phosphatase, protease,peroxidase and RNase) were detected on the Sephadex chromatogramsof leaf extracts. An UV-absorbing fraction, Fr. I-2, (immunochemicallydesignated as Pr. Imm-I) corresponded to the known "FractionI " of S. G. WILDMAN. The contents of the three antigenic components,Imm-a, Imm-b and Imm-c, having estimated molecular weights of1 to 2 105, showed significant fluctuations during growthof the leaves. Peroxidase and another antigenic component (Imm-f)of smaller molecular weight showed increase with age of theleaves. Properties of the protein components thus detected wereinvestigated. (Received May 11, 1967; ) |
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