Auxin-induced H+-pump stimulation does not depend on the presence of epidermal cells in corn coleoptiles |
| |
Authors: | Winfried Stefan Peters Ursula Richter Hubert H. Felle |
| |
Affiliation: | (1) Institut für allgemeine Botanik und Pflanzenphysiologie, Senckenbergstrasse 17-21, W-6300 Giessen, Federal Republic of Germany |
| |
Abstract: | Cell-wall acidification and electrical reactions (depolarization and hyperpolarization) are typical auxin responses in maize (Zea mays L.) coleoptiles. In an attempt to test the role of the outer epidermis in these responses, they have been measured and compared in intact and peeled coleoptile fragments. To exclude interactions between parenchymal and epidermal cells, the coleoptile pieces were completely stripped of their outer epidermis. This preparation was monitored by means of a scanning electron microscope. When externally applied indole-3-acetic acid was tested, we found that neither cell-wall acidification nor the electrical membrane responses depended on the presence of intact epidermal cells.Abbreviations IAA Indole-3-acetic acid - MES 2-[N morpholino-ethane-sulfonic acid - TRIS 2-Amino-2-hydroxymethyl-1,3-propanediolWe thank Kuki Kaethner for her excellent technical assistance. This work was supported by the Hessische Graduiertenförderung and the Deutsche Forschungsgemeinschaft. |
| |
Keywords: | Auxin (tissue specifity) Cell wall pH Coleoptile Membrane potential Zea (auxin action) |
本文献已被 SpringerLink 等数据库收录! |