小鼠体内转染CH50多肽真核表达载体趋化免疫细胞在肿瘤治疗中的作用

目的 研究体内转染表达CH50多肽对免疫细胞的趋化，聚集作用，探讨趋化聚集的免疫细胞增强抗肿瘤以及化疗后抗肿瘤的免疫力。方法 分别在小鼠后肢肌肉，接种肿瘤组织或化疗的肿瘤组织内注射pCH510质粒进行转染，取转染部位组织制备石蜡切片，观察免疫细胞的聚集情况，并观察转染pCH510及联合化疗对肿瘤的治疗作用。结果 正常小鼠后肢肌肉转染pCH510后，注射部位有大量免疫细胞聚集，其中主要是巨噬细胞，淋巴细胞，中性粒细胞，聚集的免疫细胞至少持续存在一周，肿瘤部位转染后，趋化聚集的免疫细胞分布于肿瘤组织周围，抑制了肿瘤细胞的生长，化疗后转染，仍有趋化聚集免疫细胞的作用。免疫细胞聚集数量相对只转染pCH510虽有所减少，但对肿瘤的抑制作用明显增强。结论 本文结果提示，在小鼠体内转染pCH510表达的CH50多肽不仅能够趋化免疫细胞，提高局部抗肿瘤的免疫力，而且与化疗联合作用在肿瘤治疗中具有更大的研究潜力。

RECRUITMENT OF IMMUNE BY CELLS TRANSFECTING EUCARYOTIC EXPRESSING PLASMID OF POLYPEPTIDE CH50 INTO MICE AND ITS ROLE IN TUMOR THERAPY

Objective To investigate the chemotactic activity of the recombinant polypeptide CH50 of human fibronectin (FN) expressed in vivo by transfecting the eucaryotic expressing plasmid pCH510, and to study whether the recruitment of immune cells increased the antitumor response, especially after chemotherapy. Method The plasmid pCH510 was injected into mouse muscle tissue, inoculated tumor tissue, and tumor tissue after chemotherapy, respectively. The recruitment of immune cells was observed and analyzed by histotomy and staining. The tumor-inhibiting effect of transfection of plasmid pCH50 alone and in combination with chemotherapy was assessed in mice.Result A great number of immune cells, mainly macrophages, lymphocytes and neutrophils, were observed at the injection site in the plasmid pCH50 transfection group, but not in the control group. The increased number of immune cells did not decrease significantly for a week. The immune cells assembled around the inoculated tumor tissue and the growth of tumor was inhibited. Plasmid pCH510 transfected into tumor after chemotherapy also produced the effect of chemotaxis on immune cells and inhibitied the growth of tumor, though the number of the recruited immune cells were less than those which were transfectied alone. Conclusion These findings suggest that the polypeptide CH50 expressed in vivo by transfecting pCH510 could increase local antitumor immunity . Gene therapy together with chemotherapy may have more advantages in cancer treatment.