Enzyme-linked immunosorbent assay for Met-enkephalin using unconjugated enkephalin as a solid phase antigen |
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| Authors: | Y Takesue Y Nakase-Kozaki |
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| Affiliation: | 1. Department of Immunology, School of Basic Medical Science, China Medical University, No. 77, Puhe Road, Shenyang 110122, China;2. Center of Biological Therapy, Cancer Hospital of China Medical University, No. 44, Xiaoheyan Road, Shenyang 110042, Liaoning Province, China;3. Department of Dermatology, No. 1 Hospital, China Medical University, No. 92, North Second Road, Shenyang 110001, China;4. Center of Teaching & Research, Liaoning University of Traditional Chinese Medicine, No. 79, Chongshan Eastern Road, Shenyang 110847, China;5. Immune Therapeutics, Inc., 37 North Orange Avenue, Suite 607, Orlando, FL 32801, USA |
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| Abstract: | This paper presents a competitive enzyme-linked immunosorbent assay for Met-enkephalin, in which there is no need for preparing any special derivative of Met-enkephalin for its optimal function as a solid-phase antigen. Immunoplate wells were first coated with free Met-enkephalin, and then incubated with antiserum and free Met-enkephalin. The antibodies bound to the solid-phase Met-enkephalin were determined by using anti-IgG conjugated to horseradish peroxidase. The detection limit of the assay was 44 fmol Met-enkephalin per well. The results also showed that Met-enkephalin bound to the solid phase can be titrated directly. |
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