Transferrin Receptor on Chick Fibroblast Cell Surface and the Binding Affinity in Relevance to the Growth Promoting Activity of Transferrin |
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Authors: | TAKAYUKI HASEGAWA EIJIRO OZAWA |
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Affiliation: | Division of Cell Biology, National Center for Nervous, Mental and Muscular Disorders, Kodaira, Tokyo 187 Japan |
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Abstract: | We examined the transferrin (Tf) receptor of chick skin fibroblasts using chick 125I-Tf. When the cells were incubated with 125I-Tf on ice, most of the cell-associated 125I-Tf was found on the cell surface; on the other hand, a large part of it was located inside the cells when incubated at 37°C. By equilibrium binding assay, the number of Tf receptors per cell was determined as 6.7 × 103. Dissociation constant was estimated to be 2.6 × 10−8 M. The binding of 125I-Tf was competitively inhibited by the addition of chick unlabeled Tf. Weaker inhibition was observed when bovine Tf was used as a competitor. Horse Tf had no effect on the binding of chick Tf. This agrees well qualitatively with chick cell growth-promoting activites of these Tfs. Removal of Fe from Tf affected the affinity for its receptors. About 5- to 10-fold higher concentrations of chick apo–Tf was needed to achieve the same degree of inhibition of 125I-Tf binding as that made by chick Fe-Tf. |
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