The Staphylococcal Pore-forming Leukotoxins Open Ca2+ Channels in the Membrane of Human Polymorphonuclear Neutrophils |
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Authors: | L Staali H Monteil DA Colin |
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Institution: | (1) Laboratoire de Toxinologie et d'Antibiologie Bactériennes, Institut de Bactériologie, Faculté de Médecine, Université Louis Pasteur, 3 rue Koeberlé, F-67000 Strasbourg, France, FR |
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Abstract: | The ability of leukotoxins secreted by Staphylococcus aureus to modify the permeability of the membrane of human polymorphonuclear neutrophils has been studied by spectrofluorometry
and appropriate fluorescent probes. This family of bicomponent leukotoxins is constituted by, at least, three pairs of proteins:
LukS-PV/LukF-PV, HlgA/HlgB, HlgC/HlgB. After binding of both components to the membrane, each pair induces influxes of divalent
cations and ethidium in polymorphonuclear neutrophils, although with different intensities. The influx of divalent cations
appears sooner than the influx of ethidium. The pathway for divalent cations is not permeable to monovalent cations (Na+, K+, ethidium+) and is blocked by Ca2+ channel inhibitors that do not block the fluxes of ethidium and monovalent cations. It is concluded that the leukotoxins
bind to a receptor linked to a divalent cation-selective channel or to the channel itself which is activated. Then, the leukotoxins
open a second pathway by insertion into the membrane and subsequent formation of aspecific pores allowing an influx of ethidium.
Received: 8 May 1997/Revised: 22 December 1997 |
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Keywords: | : Human polymorphonuclear neutrophil— Calcium channel— Leukotoxin— Pore-forming toxin— Staphylococcus aureus— Spectrofluorometry |
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