Cold Lability of Bacillus cereus Bacteriophage CP-51 |
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Authors: | Curtis B. Thorne and Stanley C. Holt |
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Affiliation: | 1Department of Microbiology, University of Massachusetts, Amherst, Massachusetts 01002 |
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Abstract: | Phage CP-51 was rapidly inactivated when stored at the usual refrigerator temperatures (2 to 4 C) and even more rapidly when exposed to 0 C. The loss in viability resulting from exposure to cold appeared to correlate with the increase in number of phage particles having contracted tails. High concentrations (0.01 M) of Mg2+, Ca2+, or Mn2+ stabilized the phage considerably, but even in the presence of these divalent cations, it was much less stable at 0 C than at 15 C. |
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