Ligand-dependent modulation of membrane phospholipid metabolism in ConA-stimulated lymphocytes |
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Authors: | Hinnak Northoff Bernd Dörken Klaus Resch |
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Institution: | Institut für Immunologie und Serologie der Universität Heidelberg, Im Neuenheimer Feld 305, 69 Heidelberg, Germany |
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Abstract: | Human peripheral lymphocytes were activated by ConA in serum-free culture medium, supplemented by BSA. Incorporation of 3H]thymidine into DNA, of 3H]uridine into RNA and of oleate or acetate into membrane phospholipids was investigated. DNA synthesis could be completely inhibited by αMM or by anti-ConA-IgG. Fab and F(ab)2 fragments of the anti-ConA were equally active. When αMM or anti-ConA was added to cultures at different times after stimulation with ConA, incorporation of 3H]thymidine into DNA (measured after 72 h) could be prevented up to 6–8 h completely and up to 20–30 h partially. Incorporation of 3H]uridine into RNA could be arrested at any time of the culture up to 40 h at the level it had reached but did not reverse to the level of unstimulated cells for a long time. In contrast, incorporation of oleate into lecithin returned to the level of unstimulated cells within 2–3 h after removal of ConA. This suggests that the activation of the phospholipid turnover in stimulated cells is a direct consequence of the presence of the mitogen at the membrane and thus may be a critical initial event in lymphocyte activation. |
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