|
|||||
|
|
The structure of aPhaseolus vulgaris cDNA encoding the iron storage protein ferritin |
|
| Authors: | Michael J Spence Michael T Henzl Peter J Lammers |
| Institution: | (1) Program in Molecular Biology, New Mexico State University, 88003 Las Cruces, NM, USA;(2) Department of Chemistry, New Mexico State University, 88003 Las Cruces, NM, USA;(3) Plant Genetic Engineering Laboratory, New Mexico State University, 88003 Las Cruces, NM, USA;(4) Present address: Department of Microbiology and Molecular Genetics, The University of Vermont, 05405-006 Burlington, VT, USA |
| Abstract: | A cDNA containing the entire coding region for the iron storage protein ferritin has been isolated from the French bean plant,Phaseolus vulgaris L. cv. Tendergreen. Ferritin protein was purified from young leaves and shoot meristem tissue and used to raise antisera in mice. A  gt11 cDNA library was constructed from seed-derived poly(A)+ RNA, and screened with the mouse anti-ferritin serum. A 1.2 kb immunopositive phage DNA insert was isolated and sequenced. The derived amino acid sequence shows substantial similarity with other ferritin sequences. The 5 untranslated region contains two out-of-frame AUG codons, a region of extreme pyrimidine composition bias and potentially stable secondary structure. |
| Keywords: | 5 |
| 本文献已被 SpringerLink 等数据库收录！ | |