Functional Annotation,Genome Organization and Phylogeny of the Grapevine (<Emphasis Type="Italic">Vitis vinifera</Emphasis>) Terpene Synthase Gene Family Based on Genome Assembly,FLcDNA Cloning,and Enzyme Assays |
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| Authors: | Diane M Martin Sébastien Aubourg Marina B Schouwey Laurent Daviet Michel Schalk Omid Toub Steven T Lund Jörg Bohlmann |
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| Institution: | 1.Michael Smith Laboratories,University of British Columbia,Vancouver,Canada;2.Wine Research Centre,University of British Columbia,Vancouver,Canada;3.Unité de Recherche en Génomique Végétale (URGV) UMR INRA 1165 - Université d'Evry Val d'Essonne - ERL CNRS 8196,Evry,France;4.Firmenich SA,Corporate R&D Division,Geneva,Switzerland |
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| Abstract: | Background Terpenoids are among the most important constituents of grape flavour and wine bouquet, and serve as useful metabolite markers
in viticulture and enology. Based on the initial 8-fold sequencing of a nearly homozygous Pinot noir inbred line, 89 putative
terpenoid synthase genes (VvTPS) were predicted by in silico analysis of the grapevine (Vitis vinifera) genome assembly 1]. The finding of this very large VvTPS family, combined with the importance of terpenoid metabolism for the organoleptic properties of grapevine berries and finished
wines, prompted a detailed examination of this gene family at the genomic level as well as an investigation into VvTPS biochemical functions. |
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