A novel fed-batch based cultivation method provides high cell-density and improves yield of soluble recombinant proteins in shaken cultures |
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| Authors: | Mirja Krause Kaisa Ukkonen Tatu Haataja Maria Ruottinen Tuomo Glumoff Antje Neubauer Peter Neubauer Antti Vasala |
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| Institution: | 1.BioSilta Oy, c/o Department of Biochemistry,University of Oulu,Finland;2.Fachgebiet Bioverfahrenstechnik, Institut für Biotechnologie, Technische Universit?t Berlin,Berlin,Germany;3.Department of Biochemistry,University of Oulu,Oulu,Finland |
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| Abstract: | Background Cultivations for recombinant protein production in shake flasks should provide high cell densities, high protein productivity
per cell and good protein quality. The methods described in laboratory handbooks often fail to reach these goals due to oxygen
depletion, lack of pH control and the necessity to use low induction cell densities. In this article we describe the impact
of a novel enzymatically controlled fed-batch cultivation technology on recombinant protein production in Escherichia coli in simple shaken cultures. |
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