An Instruction on the In Vivo Shell-Less Chorioallantoic Membrane 3-Dimensional Tumor Spheroid Model |
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| Authors: | Nzola De Magalhães Lih-Huei L Liaw Michael Berns |
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| Institution: | (1) Biomedical and Chemical Engineering, Rochester Institute of Technology, Rochester, NY, USA;(2) Beckman Laser Institute and Medical Clinic, University of California Irvine, Irvine, CA, USA |
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| Abstract: | The traditional shell chicken chorioallantoic membrane (CAM) model has been used extensively in cancer research to study tumor
growth and angiogenesis. Here we present a combined in vivo tumor spheroid and shell-less CAM three-dimensional model for
use in quantitative and qualitative analysis. With this model, the angiogenic and tumorigenic environments can be generated
locally without exogenous growth factors. This physiological model offers a stable, static and flat environment that features
a large working area and wider field of view useful for imaging and biomedical engineering applications. The short experimental
time frame allows for rapid data acquisition, screening and validation of biomedical devices. The method and application of
this shell-less model are discussed in detail, providing a useful tool for biomedical engineering research. |
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| Keywords: | Chicken chorioallantoic membrane CAM Spheroid Shell-less Biomedical Tumor |
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