Autometallographic enhancement of the Golgi-Cox staining enables high resolution visualization of dendrites and spines |
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Authors: | Dariusz Orlowski Carsten R Bjarkam |
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Institution: | (1) Institute of Anatomy, The Faculty of Health Sciences, Aarhus University, Wilhelm Meyers Alle, Building 1233/1234, 8000 Aarhus C, Denmark;(2) Department of Neurosurgery, Aarhus University Hospital, Norrebrogade 44, Building 10, 8000 Aarhus C, Denmark |
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Abstract: | We present a method for autometallographic (AMG) enhancement of the Golgi-Cox staining enabling high resolution visualization
of dendrites and spines. The method is cheaper and more flexible than conventional enhancement procedures performed with commercial
photographic developers. The staining procedure is thoroughly described and we demonstrate with qualitative and quantitative
data, how histological tissue sectioning, Golgi-Cox immersion time and different AMG enhancement length may influence the
staining of dendrites and spines in the rat hippocampus. The described method will be of value for future behavioural-anatomical
studies, examining changes in dendrite branching and spine density caused by brain diseases and their subsequent treatment. |
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Keywords: | Hippocampus Neurohistology Silver staining Staining method Staining procedure |
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