Measurement of carvedilol in plasma by high-performance liquid chromatography with electrochemical detection |
| |
Authors: | Machida Maiko Watanabe Masato Takechi Shigeru Kakinoki Shigeo Nomura Akikazu |
| |
Affiliation: | Department of Pathophysiology, Hokkaido College of Pharmacy, 7-1 Katsuraoka, Otaru 047-0264, Japan. |
| |
Abstract: | Carvedilol is a beta/alpha1-adrenoceptor blocker. A sensitive method for measuring plasma levels of carvedilol in human administrated low doses is needed since its plasma concentration is low. We measured carvedilol and carvedilol M21-aglycon using high-performance liquid chromatography (HPLC) with electrochemical detection. The amperometric detector was operated at 930 mV versus Ag/AgCl. Mean coefficients of variation (n = 5) for carvedilol and M21-aglycon were 4.0 and 7.7% (intra) and 6.1 and 6.7% (inter), respectively. The lower limit of quantification for each analyte was 0.10 ng/ml (signal-to-noise ratio = 3). This lower limit of quantification for carvedilol was sufficient for clinical use. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|