Purification of recombinant Bacillus cereus ResD-ResE proteins expressed in Escherichia coli strains

Recombinant E. coli strains expressing the Bacillus cereus ATCC 14579^T resD and resE genes fused with the ubiquitin gene were constructed, and purification of the ResD and ResE proteins was performed. The approach used in the study allowed us to increase the protein yield of the electrophoretic homogeneous ResD and ResE proteins without denaturation steps up to 150 mg per gram of wet cell weight.