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Constructing a genetic linkage map and mapping quantitative trait loci for skeletal traits in Japanese flounder |
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| Authors: | Yi Liu Yongxin Liu Yingjie Liu Xiaoyan Zhang Fei Si Zhaohui Sun Guixing Wang Yufen Wang Runqing Yang Haijin Liu |
| Institution: | 1. Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao, 066100, China 2. Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture, Guangzhou, 510380, China 3. Research Centre for Biotechnology, Chinese Academy of Fishery Sciences, Beijing, 100141, China 4. Research Centre for Fisheries Recourse and Environment, Chinese Academy of Fishery Sciences, Beijing, 100141, China |
| Abstract: | A genetic linkage map of Japanese flounder was constructed using 165 doubled haploids (DHs) derived from a single female. A total of 574 genomic microsatellites (type II SSRs) and expressed sequence tag (EST)-derived markers (EST-SSRs) were mapped to 24 linkage groups. The length of linkage map was estimated as 1270.9 centiMorgans (cM), with an average distance between markers of 2.2 cM. The EST-SSRs were used together with type II SSR markers to construct the Japanese flounder genetic linkage map which will facilitate identify quantitative trait locus (QTL) controlling important economic traits in Japanese flounder. Thus, twelve skeletal traits at 2 years of age were measured for all DHs. Forty-one QTLs were detected on 14 linkage groups and totally account for a small proportion of phenotypic variation (4.5 to 17.3%). Most of QTLs detected distribute on linkage groups 5 (9 QTLs), 8 (9 QTLs), 9 (5 QTLs) and 20 (4 QTLs), in which, some QTLs perform the pleiotropy. |
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