Rapid polymerase chain reaction amplification using intact bacterial cells |
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Authors: | A K Joshi V Baichwal G F Ames |
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Affiliation: | Division of Biochemistry and Molecular Biology, University of California, Berkeley 94720. |
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Abstract: | We have demonstrated that efficient polymerase chain reaction amplifications from chromosomal DNA can be carried out using whole bacterial cells as the starting material. Cells from the liquid or solid cultures can be used directly, without any pre-treatment, thus eliminating the need for DNA isolation. |
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