单相和两相发酵体系中Methylomonas Z201细胞的生长和环氧丙烷的合成

研究了单相和两相发酵体系中甲基单胞菌（Methylomonas）Z201细胞的生长和环氧丙烷的合成。在单相发酵体系中，底物丙烯和产物环氧丙烷抑制细胞生长，水相中环氧丙烷的浓度达到13mmol／L。在两相发酵体系中，十六烷作为生长底物甲烷以及反应底物丙烯和分子氧的“储器”，减小了丙烯对细胞生长的抑制作用，水相和十六烷相中环氧丙烷的浓度分别达到1．7mmol／L和2．6mmol／L。同休止细胞相比，单相和两相发酵体系中辅酶NADH的原位再生使生长细胞的操作稳定性显著提高，尤为两相体系为甚。

GROWTH OF METHYLOMONAS Z201 CELLS AND PRODUCTION OF EPOXYPROPANE MONOPHASIC AND BIPHASIC FERMENTATION SYSTEMS

The growth of Methylomonas Z201 cells and production of epoxypropane in money and biphasic fermentahon systems were studied. In monophasic fermentation systems, the inhibitions of propene and epoxypropane on the growth of Methylomonas Z201 cells were observed and the concentration of epoxypropane reached 1.3 mmol /L. In biphasic fermentation systems, hexadecane acted as the "reservoir" of growth substrate (methane) and reactants (propene, molecular oxygen), the decrease of propene and epoxypropane in aqueous phase reduced the ichbihon effect of propene and epoxypropane on the growth of cells, the concentalons of epoxypropane in both wader Phase and hexadecane phase reached l.7mmol / L and 2.6 mmol / L. In both monophasic and biphasic fermentation systems, the operational stability of cells was enhanced compared tO that of reshng cells.