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Oxidation of Two Hydroxylated Ochratoxin A Metabolites by Alcohol Dehydrogenase |
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| Authors: | Christian Syvertsen and Fredrik C Strmer |
| Institution: | Christian Syvertsen and Fredrik C. Størmer |
| Abstract: | (4R)-4-hydroxyochratoxin A, (4S)-4-hydroxyochratoxin A, and 10-hydroxyochratoxin A, all formed from ochratoxin A, were incubated with alcohol dehydrogenase in the presence of NAD. Only (4R)-4-hydroxyochratoxin A and 10-hydroxyochratoxin A acted as substrates for the enzyme. Km and turnover number for 10-hydroxyochratoxin A were 110 μM and 0.1 s−1, respectively. |
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