Somatic embryogenesis and plant regeneration from leaf tissue of jojoba |
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Authors: | Hamama L. Baaziz M. Letouzé R. |
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Affiliation: | (1) Laboratoire de Recherches en Physiologie Végétale (LRPV), des Pays de la Loire 16, Boulevard Lavoisier, 49045 Angers Cedex 01, France;(2) Faculté des Sciences-Semlalia, Département de biologie, Laboratoire de Biochimie et Amélioration des Plantes (BAP), B.P. 2390, 40000 Marrakech, Maroc |
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Abstract: | A protocol was developed for the induction, maturation and germination of somatic embryos from leaf tissue of jojoba [Simmondsia chinensis (Link) Schneider]. Explants were placed on their adaxial sides in Petri dishes and maintained in darkness on half-strength Murashige and Skoog basal medium (MS/2). Combinations of 2,4-dichlorophenoxyacetic acid (1.35–4.52 μM) with 6-benzylaminopurine (1.33–4.43μM) and 2 synthetic cytokinins, N-(2-chloro-4pyridyl)-N′-phenylurea (1.21–4.03μM) or (E)-6-[3-(trifluoromethyl)-but-2-enylamino] purine (1.11–3.71μM) resulted in formation of embryogenic cultures and somatic embryos. After two 30-day subcultures, embryogenic cultures were transferred onto MS/2 medium supplemented with different auxins and cytokinins. Somatic embryo maturation, germination and plantlet formation were achieved using 1-naphthaleneacetic acid (3.75μM) or indole-3-butyric acid (3.44μM) in combination with BA (0.44 or 1.33μM) or F3iP (0.37 or 1.11μM). Histology confirmed each stage of development. This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | plant regeneration Simmondsia chinensis somatic embryogenesis |
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