iTRAQ-based proteomic study of the effects of microcystin-LR on medaka fish liver |
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Authors: | Malécot Mélodie Marie Arul Puiseux-Dao Simone Edery Marc |
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Affiliation: | CNRS, FRE 3206 Molécules de communication et adaptation des microorganismes, Département Régulations, développement et diversité moléculaire, Muséum national d'Histoire naturelle, Paris, France. |
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Abstract: | Microcystins are cyanotoxins that occur in ground water and thus pose a potential health risk. Microcystin-LR (microcystin-leucine-arginine) is a potent hepatotoxin, and is suspected of being a tumour promoter. Poisoning with this toxin causes several dysfunctions in hepatocytes by inhibiting protein phosphatases 1 and 2A, and notably produces oxidative stress, disrupts the cytoskeleton, and deregulates mitogen-activated protein kinase pathway. Medaka fish (Oryzias latipes) was chosen as a model for studying the effects of this cyanotoxin on liver proteins using a gel-free approach, iTRAQ. Fish were gavaged with microcystin-LR. Two hours later, 325 proteins could be identified by Scaffold Q+ and 32 proteins revealed statistically significant variations above a ∣0.2∣ threshold of log(2) ratio by comparison with control. These proteins are mostly involved in the translation and maturation of proteins, lipid metabolism and detoxification. Notably, apolipoproteins are deregulated which indicates a possible alteration of chylomicron-mediated transport. |
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Keywords: | Animal proteomics iTRAQ Lipids Liver Microcystin Oryzias latipes |
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