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Effect of pH on lipid accumulation by an oleaginous yeast:Rhodotorula glutinis IIP-30
Authors:V. Johnson  M. Singh  V. S. Saini  V. R. Sista  N. K. Yadav
Affiliation:(1) the Microbiology Department School of Sciences, Gujarat University, 380 009 Ahemadabad, India;(2) the Biotechnology Laboratory, Research Centre, Gujarat State Fertilizers Company, Ltd, 391 750 Baroda, India;(3) the Applied Biology Laboratory, Research Centre, Indian Petrochemicals Corporation Ltd, 391 345 Baroda, India;(4) Ptizer Ltd., 178 Industrial Area, 160 002 Chandigarh, India;(5) the Biotechnology Laboratory, Indian Institute of Petroleum, 248 005 Deharadun, India
Abstract:Maximum lipid production (66% w/w dry wt) inRhodotorula glutinis IIP-30 utilizing glucose in a fed-batch fermentation under N-limiting conditions at 30°C, was at pH 4. At pH 3, 5 and 6, the lipid contents were 12%, 48% and 44%, respectively. There was only a small change in the fatty acid profile over the pH range examined, although the ergosterol content decreased by a third as the pH increased.
Keywords:Lipid  oleaginous  pH effect  Rhodotorula glutinis  yeast
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