Two routes for synthesis of phosphoenolpyruvate from C4-dicarboxylic acids in Escherichia coli |
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Authors: | E J Hansen E Juni |
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Affiliation: | Department of Microbiology The University of Michigan Ann Arbor, Michigan 48104 USA |
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Abstract: | Mutants of defective in both phosphoenolpyruvate carboxykinase and phosphoenolpyruvate synthetase are unable to use C4-dicarboxylic acids such as succinate and malate as carbon and energy sources for growth. Revertants that have restored function for either one of these enzymes can grow in a malate-mineral medium, but at a reduced rate compared with the growth of wild-type cells. appears to use two pathways for synthesis of phosphoenolpyruvate from C4-dicarboxylic acids. One of these involves decarboxylation of oxalacetate catalyzed by phosphoenolpyruvate carboxykinase. The second pathway makes use of the combined action of malic enzyme and phosphoenolpyruvate synthetase. |
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