Changing patterns of the IL-2/IL-2R pathway of lymphocyte activation following exposure to Plasmodium falciparum products: a study with squirrel monkey peripheral blood mononuclear cells |
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Authors: | Olivier Garraud |
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Affiliation: | Laboratoire d'Immunologie Parasitaire, Institut Pasteur de Guyane BP 6010, Cayenne, French Guiana |
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Abstract: | Abstract Squirrel monkeys are experimental hosts useful for studies on human malaria. In the present work, in vitro lymphocyte reactivity was measured by proliferation in the presence of Plasmodium falciparum -derived products, and found to depend on the previous malarial status of the animals and upon the source and/or the nature of the P. falciparum -derived material. Special attention was given to the IL-2/IL-2R pathway of lymphocyte activation. Culture supernatant from P. falciparum -parasitized erythrocytes exerted an inhibitory effect towards T lymphocytes obtained from P. falciparum -non-immune squirrel monkeys, when activated, for instance, by PHA. These lymphocytes did not incorporate tritiated thymidine (neither they did proliferate) although they expressed IL-2 α and β binding chains and secreted IL-2 (or at least TCGF). This inhibitory effect could not be rescued by the addition of rhIL-2, although the assayed lymphocytes could retain the ability to continue their cell cycle progression and divide after removal of the P. falciparum -derived inhibitory product(s). The incidence of anti-mitogenic molecules which impair the IL-2/IL-2R pathway of lymphocyte activation in malaria related processes is discussed. |
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Keywords: | Saimiri sciureus Lymphocyte activation IL-2/IL-2R Anti-mitogenicity Plasmodium falciparum products |
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