Improvement of tissue culture,genetic transformation,and applications of biotechnology to Brassica |
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Authors: | Seyed Ali Ravanfar Vladimir Orbovic Mahdi Moradpour Maheran Abdul Aziz Ratna Karan Simon Wallace |
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Institution: | 1. Department of Agronomy, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611-0300, USA;2. Citrus Research and Education Center-University of Florida/IFAS, Lake Alfred, FL 33850, USA;3. Laboratory of Plantation Crops, Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia;4. Citrus Research and Education Center-University of Florida/IFAS, Lake Alfred, FL 33850, USA;5. Laboratory of Plantation Crops, Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia;6. Department of Biology, University of Iowa, Iowa City, IA 52242-1324, USA |
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Abstract: | Development of in vitro plant regeneration method from Brassica explants via organogenesis and somatic embryogenesis is influenced by many factors such as culture environment, culture medium composition, explant sources, and genotypes which are reviewed in this study. An efficient in vitro regeneration system to allow genetic transformation of Brassica is a crucial tool for improving its economical value. Methods to optimize transformation protocols for the efficient introduction of desirable traits, and a comparative analysis of these methods are also reviewed. Hence, binary vectors, selectable marker genes, minimum inhibitory concentration of selection agents, reporter marker genes, preculture media, Agrobacterium concentration and regeneration ability of putative transformants for improvement of Agrobacterium-mediated transformation of Brassica are discussed. |
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Keywords: | Plant biotechnology Brassica gene transformation Agrobacterium tissue culture |
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