Mutants ofBacillus megaterium with altered synthesis of an exocellular neutral proteinase |
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Authors: | H Kučerová L Váchová J Chaloupka |
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Institution: | (1) Department of Cell Physiology, Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20 Prague 4 |
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Abstract: | Germinated spores ofBacillus megaterium were mutagenized with ethyl methanesulphonate and spread on test agar with caseinate. Colonies with altered proteolytic zones
or morphology were isolated and tested in liquid media. The mutants can be divided into four groups: A) those producing more
proteinase in both growth and sporulation media, B) those producing the same amount of the enzyme in growth medium but higher
amount in sporulation medium, C) those producing less proteinase in the growth medium and more in the sporulation one, D)
those producing less or no enzyme. Clones of the first three groups were phenotypically asporogenic. All mutants producing
more enzyme during growth retained their sensitivity to repression by amino acids. Isolation of mutants of types B) and C)
supports the idea of differences in the control of proteinase synthesis during growth and during sporulation. |
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