白藜芦醇对大鼠局灶性脑缺血再灌注的治疗作用

目的:观察白藜芦醇对大鼠局灶性脑缺血再灌注损伤的治疗作用及可能的机制。方法:将SD大鼠随机分为2组:对照组(n=16),白藜芦醇组(n=16)。对照组再灌注即刻腹腔给予0.5 ml生理盐水,白藜芦醇组再灌注即刻腹腔给予20 mg/kg白藜芦醇。再灌注22小时后,进行神经功能学评分、脑梗死容积测定,用分光光度仪测定脑组织溶浆中SOD、MDA和MPO的含量。结果:再灌注22小时后,白藜芦醇治疗组可以改善大鼠神经功能学评分和降低脑梗死面积(P<0.05),同时可以增加脑组织溶浆中SOD的活性,降低MDA和MPO的含量。结论:白藜芦醇通过减轻白细胞的浸润、提高自由基的清除率对大鼠局灶性脑缺血再灌注损伤发挥治疗作用。

The Treatment Effect of Resveratrol on Focal Cerebral Ischemic Rat

Objective: To investigate the effect of resveratrol on rats with focal cerebral ischemia reperfusion injury and the possible mechanism.Methods: The SD rats were randomly divided into two groups: control group(n=16) and resveratrol group(n=16).The rats in the control group were injected with 0.5 ml saline intraperitoneal,while rats in the resveratrol groups were given 20 mg/kg resveratrol after perfusion.The neurobehavioral scores,infarct volumes,the change of MPO,SOD and MDA were detected 22 hours after reperfu-sion.Results: Resveratrol treatment could improve neurobehavioral score and reduce infarct volumes after 22 h of reperfusion(P< 0.05).Meanwhile,it could increase brain soluble SOD activity,and decrease the MDA and OMP content.Conclusion: Resveratrol play a thera-peutic role on rats with focal cerebral ischemia reperfusion injury by reducing leukocyte infiltration and increasing free radical scavenging rate.