次声作用对大鼠血浆SOD、MDA、NO 水平的影响*

目的:通过观察16 Hz/130 dB次声作用大鼠不同时间其血浆超氧化物歧化酶(SOD)、丙二醛(MDA)、一氧化氮(NO)水平的变化,揭示次声作用对大鼠氧化应激损伤的特点。方法:80只SD大鼠随机分为次声作用1、7、14、21和28 d组及对应的假暴露组,每组8只大鼠。次声暴露组每日定时在次声舱内接受16 Hz/130 dB次声作用2h,连续1、7、14、21和28 d,假暴露组除不施加次声作用外,其在次声舱中放置的时间、次数、采样时间点均和次声暴露组相同。次声暴露后即刻测定大鼠血浆SOD、MDA、NO水平,测得的结果与假暴露组进行比较。结果:与假暴露组相比,大鼠血浆SOD活力在次声作用后显著降低(P<0.05),且随次声作用时间延长,SOD活力逐渐降低;大鼠血浆MDA含量在次声作用后显著升高(P<0.05),且随次声作用时间延长,MDA含量逐渐升高;大鼠血浆NO含量在次声作用7、14 d时显著降低(P<0.05),1、21和28 d时无统计学差异(P>0.05)。结论:次声作用可引起大鼠氧化应激损伤,损伤的程度与次声暴露时间有关。

Effects of Infrasound on the Levels of SOD, MDA and NO in Rat Plasma*

Objective: To observe the effects of infrasound on the levels of SOD,MDA and NO in rat plasma.Methods: 80 SD rats were randomly divided into five sham groups and five infrasound exposed groups(n=8).The rats in exposed groups were exposed under infrasound of frequency at 16 Hz,sound pressure level at 130 dB for 1,7,14,21 and 28 d,2 h per day,the rats in sham groups were treat-ed the same except infrasound exposure.The levels of SOD,MDA and NO in plasma were measured immediately after final exposure.Results: Comparing to sham groups,the activety of SOD in rat plasma decreased significantly after infrasound exposure for 1,7,14,21 and 28 d(P<0.05),the content of MDA in rat plasma increased significantly after infrasound exposure for 1,7,14,21 and 28 d(P<0.05),the content of NO in plasma decreased significantly at 7 d(P<0.05)and 14 d(P<0.05),at 0,21 and 28 d normally(P>0.05).Conclusion: Infrasound could induce oxidative stress in a time-dependent manner in rats.