退变腰椎间盘组织细胞凋亡相关蛋白酶Caspase-10 的表达研究

目的:探讨凋亡相关蛋白酶Caspase-10在腰椎间盘退变中的表达。方法:45例腰椎间盘突出症病人按年龄分为青年、中年和老年患者组,5例青少年型特发性脊柱侧弯病人作为对照组。患者组标本均为术中所取出L4/5新鲜椎间盘髓核组织,对照组标本均为术中所取出L2/3新鲜椎间盘髓核组织。采用TUNEL法和免疫组织化学S-P法,分别检测腰椎间盘髓核中的凋亡阳性细胞率及Caspase-10的表达情况。结果:对照组髓核内TUNEL阳性细胞率为(17.80±0.62)%。青年、中年、老年患者组髓核内TUNEL阳性细胞率分别为(45.71±2.05)%、(53.65±2.93)%和(68.39±4.33)%。四个组两两之间差异均有统计学意义(P<0.05)。对照组髓核内Caspase-10染色阳性细胞率为(32.60士1.64)%。青年、中年、老年患者组髓核内Caspase-10染色阳性细胞率分别为(50.67士2.89)%、(63.12士4.61)%和(75.28士4.26)%。四个组两两之间差异均有统计学意义(P<0.05)。髓核内Caspase-10染色阳性细胞率与TUNEL阳性细胞率和年龄之间均呈正相关(P<0.01)。结论:退行性变腰椎间盘组织中凋亡细胞率以及Caspase-10的表达均高于正常椎间盘组织,年龄是细胞凋亡的重要影响因素,细胞凋亡以及Caspase-10的表达上调可能在腰椎间盘退行性变的发生和发展中发挥重要作用。

Research on Expression of Apoptosis-related Protease Caspase-10 in Lumbar Disc Degeneration

Objective: To investigate the expression of apoptosis-related protease Caspase-10 in lumbar disc degeneration.Methods: 45 patients with lumbar disc herniation are divided into young,middle-aged and old patient group.5 adolescent idiopathic scoliosis patients act as control.L4/5 fresh nucleus pulposus were removed from the patient group;L2/3 fresh nucleus pulposus were removed from the control group.The rate of apoptotic cells and the expression of Caspase-10 were detected by TUNEL method and immunohistochemistry SP method.Results: The rate of TUNEL positive cells in the control group is(17.80 ± 0.62)%.It is(45.71 ± 2.05)% in the youth patient group,(53.65 ± 2.93)% in the middle age patient group and(68.39 ± 4.33)% in the old patient group.Differences between each of four groups were significant(P<0.05).The Caspase-10 positive cell rate is(32.60 ± 1.64)% in the control group,(50.67±2.89)% in the youth patient group,(63.12 ±4.61)% in the middle age patient group and(75.28 ± 4.26)% in the old patient group.Differences between each of four groups were significant(P <0.05).Both the Caspase-10 positive cell rate and TUNEL-positive cell rate have positive correlation with age(P<0.01).Conclusions: Apoptosis and the expression of Caspase-10 protein are higher in the disc degeneration tissues than normal.Age is an important factor of apoptosis.Apoptosis and Caspase-10 may play an important role in the lumbar intervertebral disc degeneration.