人脑胶质瘤中热休克蛋白70、Caspase-3 的表达及临床意义

目的:探讨热休克蛋白70、caspase-3在人脑胶质瘤中的表达和临床意义。方法:收集2008年7月～2010年12月汕头大学医学院第二附属医院神经外科手术获取的人脑胶质瘤组织标本35例,另选择20例脑外伤手术中切除的正常脑组织标本作为对照组。采用EnVision免疫组化方法检测脑胶质瘤组织和正常脑组织中热休克蛋白70、caspase-3的表达,并分析其与脑胶质瘤组织临床病理特征之间的关系。结果:HSP70在胶质瘤组和对照组中的阳性表达率分别为74.3%和25.0%,胶质瘤组的HSP70表达明显高于对照组(P<0.05)。caspage-3在胶质瘤组和对照组中的阳性表达率分别为34.3%和80.0%,胶质瘤组的caspage-3阳性表达率均明显低于对照组(P<0.01)。HSP70和caspase-3的阳性表达与胶质瘤的病理学分级、术后复发情况密切相关(P<0.05)。相关分析表明,胶质瘤组织中HSP70阳性表达率和caspase-3表达呈负相关(r=-0.568,P<0.05)。结论:胶质瘤组织中HSP70呈高表达,而caspase-3表达下调,两者均在胶质瘤浸润、复发等恶性演进过程中发挥重要作用;HSP70可能通过某些途径抑制caspase-3表达来抑制胶质瘤恶性细胞的凋亡发生。

The Expression and Clinical Significance of HSP70 and Caspase-3 in Gliomas

Objective: To investigate the expression and clinc significance of HSP70 and caspase-3 in gliomas.Methods: The expression of HSP70 and caspase-3 protein was detected with immunohistochemical EnVision plus non-hiotin technique in 35 cases of gliomas and 20 cases of normal cerebral tissue.The correlation with clinicopathologic characteristic were analyzed.Results: The positive expression rates of HSP70 protein in gliomas(74.3%) were significantly higher than that in normal cerebral tissue(25.0%) and the positive expression rates of caspase-3 protein in gliomas(34.3%) were significantly lower than that in normal cerebral tissue(80.0%)(P<0.01).The expression of HSP70 and caspase-3 were positively related to tumor clinica1 stage and recurence(P<0.01).HSP70 expression was negatively correlated with caspase-3(r=-0.568,P<0.05).Conclusion: HSP70 showed high expression and the expression of caspase-3 decreased in gliomas.HSP70 and caspase-3 play an important role in metascasis and recurence in gliomas.HSP70 inhibites apoptosis control of tumor cells of gliomas through inhibiting the expression of caspase-3.