光照对麻醉大鼠海马节律的影响

目的:研究光照刺激对麻醉大鼠海马节律的影响。方法:对乌拉坦麻醉大鼠,在海马中植入电极,通过神经信号放大器记录场电位。重复对大鼠进行视觉刺激,刺激10s,并给予20s的恢复期。与经典掐尾实验进行对比。结果:乌拉坦麻醉大鼠的掐尾实验能够将海马场电位从"大不规则波"(LIA)变成theta波。LIA到theta的转变通常只需1s,掐尾结束后3s后theta返回LIA。光刺激造成了在5-8s后LIA消失,光刺激撤离后4-6sLIA返回,并没有引发theta。结论:本文结果提示视觉通路与触觉通路有不同的优先级和行为特性。

Effects of Light Stimulation on the Hippocampal Rhythm of Anaesthetized Rats

Objective: To investigate the effects of light stimulation on hippocampal rhythm of anaesthetized rats.Methods: The rats were anaesthetized with urethane and implanted with electrodes in the hippocampus,from which neural local field potentials(LFPs) were recorded.Light stimulation were applied to the rats repetitively with 10s duration and additional 20s delay for recovery,which was compared with the classical tail-pinch experiment.Results: The tail-pinch of urethane-anesthetize rat could change the LFPs in hippocampus from large irregular activity(LIA) into theta wave,which indicated that a sensory input could cause the animal being ’active’.The transition of LIA to theta wave is within 1s,and that theta wave back to LIA is approximately 3s after pinch’s removal.We found that light can eliminate LIA but not elicit theta wave,with much longer delay,5-9s for LIA disappearing after light onset and 4-6s for LIA’S back.Conclusions: This may suggest that under urethane-anesthetized condition visual input is less sensitive and with lower priority comparing with tactility.