四种口腔修复材料对细胞的毒性分析

目的:研究纯钛、钛合金、钴铬合金和镍铬合金四种非贵金属口腔修复材料对L-929成纤维细胞的毒性作用。方法:应用口腔修复材料制备材料浸提液处理培养L-929细胞,采用AnnexinV-FITC试剂盒比较细胞凋亡水平改变,采用Western blot检测细胞凋亡相关基因的表达。结果:纯钛与钛合金诱导L-929细胞凋亡的水平与对照组没有统计学差异(P>0.05);钴铬合金和镍铬合金材料浸提液可引起L-929细胞凋亡增加(P<0.05)。结论:纯钛与钛合金材料对口腔黏膜细胞的毒性作用相较钴铬合金和镍铬合金材料低,更具安全性。

Toxicity Analysis of Four Non-noble Metal Oral Cavity Repair Materials on Oral Epithelium Cell

Objective: To compare the toxicity on oral epithelium cell among four non-noble oral repair materials including pure Ti,Ti alloy,Co-Cr alloy and Ni-Cr alloy materials.Methods: We used cell culture,AnnexinV-FITC kit and Western blot to evaluate the cell apoptotic levels induced by different materials.Results: Ti and Ti alloy material could not induce detectable apoptosis in oral epithelium cell line L-929(P <0.05),while Co-Cr alloy and Ni-Cr alloy could cause increased cell apoptosis compared with controls(P < 0.05).Conclusions: Ti and Ti alloy materials cause less toxicity on oral epithelium cells than Co-Cr alloy and Ni-Cr alloy materials,which proved to be safer in clincial use.