| 噬菌体抗体库筛选技术 |
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| 引用本文： | 高鹏 胡立勇 钱钰 徐晨. 噬菌体抗体库筛选技术[J]. 现代生物医学进展, 2012, 12(23): 4577-4583 |
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| 作者姓名： | 高鹏 胡立勇 钱钰 徐晨 |
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| 作者单位： | 广东药学院基础学院;中国药科大学药学院;北京三元基因工程有限公司 |
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| 摘 要： | 在过去的20年中,噬菌体抗体库筛选技术被广泛的应用于抗体筛选、疾病治疗,临床诊断以及基础研究之中。在该技术的应用过程中,快速有效的筛选出最适合的单克隆抗体并进行可靠和高效的数据管理和分析是十分重要的。文中总结了近年来噬菌体抗体库的高通量筛选方法并且对数据管理做了简要介绍。
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| 关 键 词： | 噬菌体展示 抗体库 筛选技术 数据管理 |
Screening Isolates from Antibody Phage-display Libraries |
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| GAO Peng,HU Li-yong,QIAN Yu,XU Chen. Screening Isolates from Antibody Phage-display Libraries[J]. Progress in Modern Biomedicine, 2012, 12(23): 4577-4583 |
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| Authors: | GAO Peng HU Li-yong QIAN Yu XU Chen |
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| Affiliation: | 1School of Basic Courses,Guangdong Pharmaceutical University,Guangzhou 510006,China; 2 School of Pharmacy,China Pharmaceutical University,Nanjing 210009,China; 3 Beijing Tri-Prime Genetic Engineering Co.Ltd,Beijing 102600,China) |
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| Abstract: | For almost 20 years,phage display antibody(Ab) libraries screening have been widely used in antibody screen,clinical diagnostic,treatment of disease and basic research areas.Rapid identification of the autofit monoclonal antibody,and reliable and efficient data management and analysis are very important in the application of this technology.This review described reported methods for high-throughput screening of antibody phage-display libraries and further more gave a brief introduction to data management. |
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| Keywords: | Phage display Antibody library Screening technology Data management |
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