| PESV 对K562 细胞BCR/ABL 融合基因及凋亡调控因子Bcl-2、Bad
表达的影响 |
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| 引用本文： | 于文俊,杨文华,杨向东,史哲新,王兴丽,郝征,张佳.PESV 对K562 细胞BCR/ABL 融合基因及凋亡调控因子Bcl-2、Bad
表达的影响[J].现代生物医学进展,2012,12(21):4015-4018. |
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| 作者姓名： | 于文俊 杨文华 杨向东 史哲新 王兴丽 郝征 张佳 |
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| 作者单位： | 1. 天津中医药大学 天津300193
2. 天津中医药大学第一附属医院 天津300193 |
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| 基金项目： | 教育部博士点基金(20091210110004) |
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| 摘 要： | 目的:探讨PESV对K562细胞BCR/ABL融合基因及凋亡调控因子bcl-2和bad表达的影响.方法:将体外培养K562细胞,经PESV处理不同时间后,流式细胞术检测细胞凋亡率,荧光定量RT-PCR检测BCR/ABL、Bcl-2、Bad mRNA水平变化.结果:与对照组相比,PESV处理后K562细胞,凋亡率增加,BCR/ABL融合基因表达降低,抗凋亡相关基因Bcl-2 mRNA表达降低,促凋亡基因Bad mRNA表达增加.结论:PESV能降低降低K562细胞BCR/ABL融合基因的表达,可能通过调节Bcl-2和Bad表达,抑制K562细胞增殖,促进其凋亡.
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| 关 键 词： | PESV K562细胞系 凋亡 BCR/ABL Bcl-2 Bad |
The Effects of PESV on the Expression of BCR / ABL Fusion Gene and
Bcl-2, Bad of Apoptosis Regulators on the K562 Cells |
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| YU Wen-jun,YANG Wen-hu,YANG Xiang-dong,SHI Zhe-xin,WANG Xing-li,HAO Zheng,ZHANG Jia.The Effects of PESV on the Expression of BCR / ABL Fusion Gene and
Bcl-2, Bad of Apoptosis Regulators on the K562 Cells[J].Progress in Modern Biomedicine,2012,12(21):4015-4018. |
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| Authors: | YU Wen-jun YANG Wen-hu YANG Xiang-dong SHI Zhe-xin WANG Xing-li HAO Zheng ZHANG Jia |
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| Institution: | 1(1 Tianjin University of Traditional Chinese Medicine,300193 Tianjin,China; 2 The First Hospital Affiliated to Tianjin Medical University,300193 Tianjin,China) |
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| Abstract: | Objective: To investigate the PESV of K562 cells BCR / ABL fusion gene and apoptosis regulators bcl-2 and bad ex-pression.Methods: K562 cells were cultured in vitro,by PESV for different times,the apoptosis rate by flow cytometry,fluorescence quantitative RT-PCR detection of BCR / ABL,Bcl-2,Bad mRNA level changes.Results: Compared with the control group,PESV treated K562 cells,apoptosis increased,BCR / ABL fusion gene reduced expression,anti-apoptotic gene Bcl-2 mRNA expression decreased,pro-apoptotic gene Bad mRNA expression.Conclusion: PESV reduced in K562 cells can reduce the BCR / ABL fusion gene,may regu-late the expression of Bcl-2 and Bad,inhibit proliferation of K562 cells and promote their apoptosis. |
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| Keywords: | PESV K562 cell lines Apoptosis BCR / ABL Bcl-2 Bad |
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