京尼平或紫外线交联制备的壳聚糖/ 藻酸盐复合支架材料比较

目的:比较京尼平或紫外线交联的壳聚糖/藻酸盐复合支架材料的降解率、孔隙率、含水量、细胞毒性以及生物力学等特性。方法:①按照交联方法不同分为:京尼平组、紫外线组。②扫描电镜下观察材料的表面结构以及检测材料的降解率、孔隙率、含水量、细胞毒性以及生物力学。结果:①紫外线组与京尼平组均表现为多孔隙结构,无明显差异。②紫外线组降解率高于京尼平组。③京尼平组与紫外线组的孔隙率差异无统计学意义,含水量差异比较有统计学意义。④两组均表现为较低的细胞毒性,良好的生物相容性。⑤京尼平交联组的生物力学特性较紫外线组显著提高。结论:京尼平交联的壳聚糖/藻酸盐复合支架材料,具有良好的生物学特性,为组织工程脊髓领域提供了非常具有潜力的材料。

Comparison of Genipin or Ultraviolet Crosslinking Prepared Chitosan/Alginate Composite Scaffold Material

Objective: To investigate genipin(GP) or ultraviolet rays(UV) crosslinking effect on chitosan/alginate composite scaffold material for tissue engineering by comparing their degradation rate,porosity,water content,cytotoxicity and biomechanics properties.Methods: ①According to different cross-linking methods,biomaterials were divided into(GP)group,(UV)group.②The surface structure of two groups was detected by scanning electron microscope(SEM).The material degradation rate,porosity,water content,cytotoxicity,and biomechanics properties were detected.Results: ①both UV group and GP group showed pore-rich structure,and there was no significant difference between the two groups.②Degradation rate of UV group was higher than that of GP group.③GP group and UV group had no significant difference in porosity.The difference of water content is meaningful in Statistics.④GP group and UV group showed low cytotoxicity,good biocompatibility.⑤Biomechanics properties of GP group were significantly improved compared with UV group.Conclusion: Genipin crosslinked chitosan/alginate composite scaffold material has good biological characteristics.It is a potential material in tissue engineering of spinal cord field.