王氏连朴饮对脾胃湿热证大鼠行为、肾上腺指数及血清Cort 的影响

目的:观察王氏连朴饮对脾胃湿热证大鼠的行为学、肾上腺指数及血清皮质醇(Cort)的调控作用。方法:将45只雄性Wistar大鼠随机分为正常对照组、模型组、王氏连朴饮(高、中、低剂量)组。利用复合因素复制脾胃湿热证动物模型。通过旷场实验观察大鼠行为学改变,称量法计算肾上腺指数,采用放射免疫法检测大鼠血清Cort的水平。结果:造模成功后大鼠行为发生改变,王氏连朴饮各剂量组对大鼠行为均有恢复作用,但各剂量组间无显著性差异;大鼠肾上腺指数增高,血清Cort升高(P<0.05),王氏连朴饮各剂量组对其均有降低作用,以中剂量组效果最好且有显著性差异(P<0.05)。结论:王氏连朴饮对脾胃湿热证大鼠行为、肾上腺指数和血清Cort有干预作用,这可能是王氏连朴饮清热祛湿的机制之一。

Regulation ofWangshi-Lianpu-Yin Decoction on Behavior, Adrenal Index and Serum Cortisol of Rats with Spleen and Stomach Damp-Heat Syndrome

Objective: To investigate the regulation effect of Wangshi-Lianpu-Yin(WLY) decoction on behavior,adrenal index and serum Cort(cortisol) of rats with spleen and stomach damp-heat syndrome(SSDHS).Methods: Forty-five male Wister rats were randomly divided into five groups: normal control group(NCG),model group(MG),WLY high(HWG),moderate(MWG) and low dosage groups(LWG).The behavior of rats was monitored with open-field test.Adrenal index were calculated by quantified method.The levels of serum Cort were detected by RIA method.Results: Experiments showed that there were changes in the behavior of rats with SSDHS.WLY decoction with various doses all had effect on the behavior of the rats,but no statistical differences could be seen between groups treated with different doses(P >0.05).Compared with normal control group,the adrenal index and serum Cort of the model group increased significantly(P <0.05).WLY decoction of different doses all reduced the adrenal index and serum Cort(P>0.05 or P<0.05),and especially the MWG had best outcome.Conclusions: WLY decoction can control the behavior,the adrenal index and serum Cort of the rats with SSDHS.This may be one of the functions of the WLY.