刺参酸性粘多糖对诱发性肝癌大鼠的干预作用及免疫功能的影响*

目的:通过建立诱发性大鼠肝癌动物模型,研究用刺参酸性粘多糖(SJAMP)对大鼠诱发性肝癌的干预作用及免疫功能的影响。方法:将雄性Wistar大鼠50只随机均分为5个组,正常对照组、模型组和3个SJAMP干预组(A组,B组和C组),模型组和SJAMP干预组灌胃0.2%DEN生理盐水溶液以诱发肝癌,同时SJAMP干预组按照不同剂量(0.175μg/g,0.35μg/g,0.7μg/g)给予SJAMP,至16周处死大鼠,取血,无菌取脾、胸腺,计算脾指数、胸腺指数。比较各组>3mm和>5mm的结节数以及最大结节的体积,计算肿瘤抑制率。贴壁法纯化巨噬细胞,用中性红吞噬实验检测巨噬细胞吞噬功能,MTT法检测巨噬细胞杀伤功能。结果:SJAMP干预组>3mm和>5mm的结节数明显少于模型组,最大结节的平均体积明显小于模型组(P<0.05);与模型组相比,SJAMP干预组脾指数和胸腺指数明显升高(P<0.05),SJAMP干预组巨噬细胞吞噬能力和杀伤功能显著提高(P<0.05)。结论:刺参酸性粘多糖对大鼠诱发性肝癌有明显的抑制作用;其机制可能是通过刺激免疫器官生长,增强机体的细胞免疫能力来实现的。

The Intervention Effect of SJAMP on Hepatocellular Carcinoma Rat Induced by Diethylnitrosamine and Impact on Immune Function

Objective: To establish animal models of Hepatocellular Carcinoma in rats and to investigate the intervention effect of Stichopus japonicus acid mucopolysaccharide(SJAMP) on Hepatocellular Carcinoma and immune function.Methods: 50 male Wistar rats were randomly divided into five groups: normal control group,model group and SJAMP intervention groups(group A B C).The model group and SJAMP intervention groups were fed with 0.2 % DEN saline solution up to the 16th weeks to induce hepatocelluar carci-noma,meanwhile they were intervened with different doses of SJAMP during the entire carcinogenesis process,then all the rats were killed.Blood samples were drawn from the abdominal aorta,the spleen and thymus were removed under sterile conditions,and the spleen index and thymus index were calculated.The number of the nodules ≥3mm and ≥5mm,the size of the largest nodule was compared between groups.The tumor inhibitory rate was calculated.Adherent purified Mφ,phagocytosing neutral red method was used to detect macrophage phagocytosis and spleen macrophage killing capability was assayed by MTT method.Results: The number of the nodules ≥3mm and ≥5mm and the mean volume of the largest nodule of SJAMP intervention groups was significantly less than that in model group(P<0.05),and the tumor inhibition of SJAMP was significantly higher than model group;Compared with model group,the spleen indices and thymus indices of SJAMP intervention groups were increased significantly(P<0.05);The phagocytosis and killing capability of Mφ of SJAMP intervention groups were all higher than those in model group(P<0.05).Conclusion: SJAMP has significantly inhibition on the occurrence of liver cancer in rats and the mechanism which may be stimulate the immune organ tissue enhance the body’s cell-me-diated immunity ability.