斑马鱼胚胎和肿瘤细胞评价细胞毒药物活性比较研究*

目的:比较斑马鱼胚胎和肿瘤细胞作为药物筛选模型的优缺点.方法:采用MTT法检测顺铂、紫杉醇、阿霉素、5-氟尿嘧啶四种药物对HL-60和Hela细胞的增殖影响；同时,观察药物对斑马鱼胚胎发育的影响.结果:阿霉素、顺铂及紫杉醇作用于HL-60及Hela细胞的IC50均显著高于作用于斑马鱼胚胎的LD50；而5-FU作用于肿瘤细胞和斑马鱼胚胎的结果与其它药物相反；四种抗肿瘤药物对斑马鱼胚胎的生长发育均有致畸作用.结论:斑马鱼胚胎作为细胞毒类药物筛选模型,对于抗微管类药物较为敏感,但对于抗代谢药敏感性较肿瘤细胞差.

Evaluation Cytotoxic Drugs Activities with Zebrafish Embryos and TumorCells *

Objective: To investigate the differences in cytotoxic drug screening with zebrafish embryos and tumor cells.Methods: Human leukemia cells(HL-60) and cervical cancer cells(Hela) were used to evaluate the cytotoxic effect of four different antineoplastics(paclitaxel,5-fluorouracil,doxorubicin and cisplatin) with MTT assay.And the impact of the four drugs on zebrafish embryos development was also obersseved.Results: The data demonstrated that the half inhibitory concentration(IC50) of paclitaxel,doxorubicin and cisplatin on cancer cells significantly exceeded the LD50 of the three drugs on zebrafish embryos.However the effect of 5-fluorouracil was opposite.And the four drugs had teratogenic effect on zebrafish embryos.Conclusion: Zebrafish and embryos as a new model for drug screening in cytotoxic drugs,is more susceptible to the inhibitor of microtubule formation and less susceptible to antimetabolites than tumor cells.