不同麻醉方法对肝癌手术患者炎性细胞因子基因表达的影响

目的:探讨不同麻醉方法对肝癌手术患者外周血炎性细胞因子基因表达的影响。方法:选择肝癌手术患者48例,随机分为单纯全麻和全麻复合硬膜外麻醉两组,在麻醉前和麻醉后4h抽取静脉血,Trizol法抽提RNA,RT-PCR检测IL-1β、IL-6、IL-8的TNF-α的mRNA表达水平。结果:麻醉前两组患者间外周血炎性细胞因子基因表达无差别,麻醉4h后全麻组外周血IL-1β、IL-6、IL-8的mRNA表达水平显著升高(P<0.05),并高于联合组(P<0.05)。结论:不同麻醉方法对细胞因子的分泌会产生不同的影响,单纯全麻将增强肝癌手术病人外周血炎性细胞因子基因的表达。

The Impact of Different Anesthesia Methods on Gene Expressionof Inflammatory Cytokines in Hepatoma Surgery Patients

Objective: To explore the effects of different anesthetic techniques on gene expression of inflammatory cytokines in patients with liver cancer surgery.Methods: 48 liver cancer surgery patients were randomly divided into two groups,general anesthesia group and general anesthesia plus epidural anesthesia group.Draw venous blood before anesthesia and 4h after anesthesia.Extract RNA by Trizol method.Then detect the IL-1β,IL-6,TNF-α and IL-8 mRNA expression levels with RT-PCR.Results: The inflammatory cytokines gene expression in peripheral blood of the two groups had no difference before anesthesia.4h after anesthesia,the mRNA expression levels of IL-1β,IL-6 and IL-8 general were significantly increased in anesthesia group(P<0.05),and much higher than the combination group(P<0.05).Conclusion: Different aesthetic methods will produce different effects on cytokine secretion.General anaesthesia could enhance inflammatory cytokine gene expression in peripheral blood of patients with liver cancer surgery.